ABSTRACT
Introduction: Diabetes mellitus is one of the five leadingcauses of death in the world.Objective: Our main goal is to estimate the effect of aqueousextract of Momordica charantia on blood glucose level inalloxan induced diabetic rats.Methodology: This experimental study was carried out atDepartment of pharmacology, Dhaka medical college, Dhakafrom July 2014 to June 2015 where the study was divided intotwo parts, Experiment-1 and Experiment-2. In Experiment-1include group A and group B. Experiment-2 include group C,group D and group E. Blood was collected from group A andgroup B on day 1 and day 15 and from group C,D and E onday 1,4 and 15 of experiment.Results: During the result in group-A (served as control andreceived normal rat diet for 14 days) the blood glucose levels(mean ± SD) were 4.90±0.50 mmol/L and 5.3±0.38mmol/L onday 1 and day 15 respectively. Percent inhibition was 4.8 andin group-B the blood glucose levels (mean ± SD) were5.00±0.36mmol/L and 5.05±0.32 mmol/L on day 1 and 15respectively. Also for in group C, the blood glucose levels(mean ± SD) were 5.31± 0.45 and 14.24±.51 on day 1 andday 15 respectively.Conclusion: From the analysis we can conclude that thataqueous extract of Momordica charantia has hypoglycaemiceffect, thus provide a rational for its use in development of newdrug require for treatment and prevention of complications ofdiabetes mellitus.
ABSTRACT
Background: Evidence shows that high density lipoproteins (HDLs) may exert multiple anti-atherogenic and anti-thrombotic effects that together decrease the risk of cardiovascular morbidity in hyperlipidaemic state. Experimental studies showed that fresh wheatgrass (Triticum aestivum Linn.) juice exerts lipid lowering effect as well as increases HDL-cholesterol level in rats. Objective: The present study was done to observe the effect of wheatgrass juice on serum HDL-cholesterol on experimentally induced dyslipidaemic rats evident by reduced HDL-cholesterol level. Materials and method: This experimental study was carried out in the Department of Pharmacology & Therapeutics, Dhaka Medical College, Dhaka, from July 2009 to June 2010. A total number of 48 healthy adult male Long Evans rats, age ranging from 2-3 months, and weighing from 140-200 grams were used for the present study. Six rats were taken in each group of total 8 groups treated differently with 1 control group. Rats of the first 5 groups were sacrificed at 29th day and their lipid profile was measured. Rests of the rats were sacrificed at 57th day and their lipid profile was measured. Results: Mean HDL-cholesterol of the group of rats treated with 10 mL/kg grass juice and 1% cholesterol diet, with 10 mL/kg grass juice and with 20 mL/kg grass juice were significantly increased (P<0.05, P<0.05, P<0.01 respectively). The mean HDL-cholesterol of the group treated with Atorvastatin for 28 days were also significantly increased (P<0.001). Conclusion: The present experiment showed that HDL-cholesterol level is increased by ingestion of wheat grass juice in experimental animals. Therefore, it provides the initial step for demonstrating cardio-protective effect of wheatgrass juice by increasing HDL-cholesterol level in blood in dyslipidaemic state.
ABSTRACT
Background: Drug can cure ailment when used rationally on the other hand they may become harmful or even may threaten a life when used irrationally. Absence of guidelines for antibiotic use, protocols for rational therapeutics and infection control committees, have led to overuse and misuse of antimicrobials even in different specialized units in hospitals. Objective: The study has been designed to get a picture of use of antibiotics in a tertiary care hospital of Bangladesh. Materials and method: Cross sectional study was conducted in the department of Medicine of Sir Salimullah Medical College and Mitford Hospital, Dhaka, Bangladesh, for 3 months from 1st January 2009 to 31st March 2009. Admitted patients of Medicine unit-1 who got antibiotics were included in the study. Total number of patients was 1563. Results: Five hundred out of 1563 patients were prescribed antibiotics (38%). Out of 500 prescriptions, 68(14%) prescriptions were found irrational. Conclusion: Continuous surveillance should be carried out to reduce the irrational use of antibiotics.